异搏定对化疗药物体外净化白血病细胞的增强效应

Effects of verapamil upon et vivo purging activity of adriamycin, vincristine, or etopside on leukemic cells

用体外克隆形成培养技术研究异搏定（ＶＰＬ）对化疗药物阿霉素（ＡＤＭ）、长春新碱（ＶＣＲ）及足叶乙甙（ＶＰ－１６）体外净化白血病细胞作用的影响，结果显示ＶＰＬ能明显提高不同浓度的３种药物体外对Ｋ５６２、Ｒａｊｉ及Ｌ－ＣＦＵ的杀伤敏感性，而不增加其对ＧＭ－ＣＦＵ的毒性。２．２０μｍｏｌ·Ｌ－１ＶＰＬ联用０．９２μｍｏｌ·Ｌ－１ＡＤＭ、０．０２５μｍｏｌ·Ｌ－１ＶＣＲ或１．７１μｍｏｌ·Ｌ－１ＶＰ－１６对Ｌ－ＣＦＵ的杀伤作用分别为不加ＶＰＬ组的１．９６、１．６５及１．９５倍，而ＧＭＣＦＵ集落存活率则无明显改变，提示ＶＰＬ能选择性提高上述化疗药物的体外净化白血病细胞效果。

Using semi-solid culture technique of clonal formation in vitro, we observed the effects of VPL upon ex vivo purging activity of ADM,VCR or VP-16 on leukemic cells. The results showed that VPL significantly enhanced the killing sensitivity of ADM, VCR Of VP-16 on K562,Raji or leukemic progenitor (L-CFU) cells,but did not increase their toxicities to GMCFU. When 2.20 μmol· L-1 VPL was combined with 0.92μmol·L-1 ADM,0.025μmol·L-1VCR or 1.70μmol·L-1VP-16 for purging,1.96, 1.65, 1. 95 times of L-CFU was spared than that of group without VPL, respectively, but the survival rate of GM-CFU was not influenced significantly, suggesting that VPL selectively enhanced the ex vivo purging effect of above chematherapeutic agents on leukemic cells.