RP－HPLC测定胰岛素滴眼液的含量

Determination of insulin in eyedrop by reversed-phase HPLC

采用反相高效液相色谱法（ＲＰ－ＨＰＬＣ）测定胰岛素滴眼液的含量。在ＯＤＳ柱上，以Ｎａ２ＨＰＯ４－．柠檬酸缓冲液（含０．０５ｍｏ１／ＬＮａ２ＳＯ４）－乙腈（６８：３２）为流动相，测得胰岛素滴眼液的平均回收率为１０５％，ＲＳＤ＝２．９６％。本法分离效果和重现性均良好，一次进样８ｍｉｎ内即完成，方法简便、迅速、准确、易行。

We report a method for determination of insulin in eyedrop by reversed-phase highperformomce liquid chromatography,Insulin was separated on a Cis column(7μm,4.6 mm X 25cm) by using 0.1 mol/L phosphate-citric acid buffer with 0.05 mmol/LNa2SO4-acetontrile(68:32)as mobile phase and detection at 280 nm。There was a good linearity ranging from 43. 06 to688. 96 u/ml of insulin Y=0.00204+1. 921X(r=0. 9997). The average recovery was 105%,RSD=2.96%(n=5).The method is simple ,rapid ,accurate and repeatable。