感觉神经植入皮瓣后再生纤维成熟过程的电镜观察

ABSTRACTOBSERVATION OF THE PROGRESS OF NERVE REGENERATION AFTER SENSORY NERVE BEING IMPLANTED INTO SKIN FLAP UNDER ELECTRON MICROSCOPE

采用兔耳大神经植入颈肩部失神经皮瓣模型，动态观察植入神经成熟过程。按神经植入皮瓣后时间，将动物分为１个月、２个月、４个月和６个月四个实验组，另设正常皮肤和未植神经对照组，每组动物４只。用电镜进行观察。结果表明，再生纤维的发育过程是：２个月再生无髓纤维，４个月再生有髓纤维。轴突直径，髓鞘厚度，有髓、无髓纤维比例均随时间延长而增大。４个月再生的轴突数达最高水平，显著超过正常，主要为无髓轴突。６个月时有髓、无髓纤维比例仍显著低于正常。

This article reported the implantation of the greater auricular nerve into the cervico-shoulderskin flap in rabbits. The progress following implantation of the nerve was continuously observed.Aecording to the time elapsed from the nerves being implanted,the experimental animals werediviued into 4 groups,4 animals in each group, in the order of 1,2, 4 and 6 months groups,re-spectively,and the normal skin and the cervico-shoulder skin flaps without nerve implantationwere used as control. The electron microscopewas the method used to observed the progress.The results showed that in the 2nd month the unmyelinated fibers first appeared , then the myeli-nated in the 4th month.The caliber of the axons , the thick ness of the myelin sheath and the ra-tio of MF/UF increased with time. In the 4th month , the number of the regenerating nervefibers(unmyelinated fibers predominant ) had achieved the highest level and significantly exceed-ed that of the normal skin. But at the 6 months.the MF/UF ratio was still for lower than nor-mal.