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尼卡地平升高小鼠胸腺细胞胞浆钙浓度(英文)

Nicardipine enhances cytoplasmic calcium concentration in mouse thymocytes in vitro
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摘要 研究尼卡地平(nicardipine,Nic)对小鼠胸腺细胞胞浆钙浓度([Ca^(2+)]_i)及增殖的影响.方法:用Fura-2掺入细胞的荧光测定法测定[ca^(2+)]_i;用[~3H]thymidine掺入法测定胸腺淋巴细胞的增殖.结果:无论在含Ca^(2+)或无Ca^(2+)介质中,Nic 1—30 μmol·L^(-1),以浓度依赖的方式升高静息胸腺细胞的[Ca^(2+)]_i.丝裂原Con A 5 mg·L^(-1)也从细胞内库释放Ca^(2+),而Nic抑制Con A引起的[Ca^(2+)]_i升高.在上述升高[Ca^(2+)]_i的浓度中,Nic不刺激静息胸腺淋巴细胞增殖,但显著抑制Con A的增殖反应.结论:Nic升高[Ca^(2+)]_i,干扰了细胞Ca^(2+)稳态,因而抑制淋巴细胞对丝裂原的反应. To study the effect of nicardipine (Nic) on the cytoplasmic free calcium concentration ([Ca2+];) in thymocytes and on the proliferation of thymus lymphocytes from mice in vitro. METHODS: The [Ca2+1 was measured by loading cells with the fluorescent dye Fura-2. The lymphocyte proliferation was determined by [3H]thymidine incorporation. RESULTS: Nic 1 - 30μmol·L-1 elevated [Ca2+ ]i of resting thymocytes with the percentages of its increase 43 %- 192 % and 12 % -148 % in the presence and absence of extracellular Ca2+ respectively. Mitogen Con A 5 mg·L-1 also released Ca2+ from intra-cellular stores. Nic decreased the enhancement of [Ca2+]i induced by Con A. The IC50 (μmol·L-1) was 26. 8 (95 % limit: 24. 8 -29- 0) and approximated to 10 in the presence and absence of extracellular Ca2+ respectively. Nic 1 - 30μmol·L-1 did not stimulate the proliferation of resting lymphocytes from mouse thymuses (P>0. 05) , but inhibited the proliferation when the lymphocytes had been stimulated by Con A (P<0. 01). CONCLUSION: Nic elevated [Ca2+]i, which decreased the response of lymphocytes to mitogen.
出处 《中国药理学报》 CSCD 1995年第6期489-492,共4页 Acta Pharmacologica Sinica
基金 National Natural Science Foundation of China, №3870891.
关键词 尼卡地平 胸腺 细胞质 淋巴细胞转化 细胞培养 nicardipine thymus gland cytoplasm calcium lymphocyte transformation cultured cells
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  • 1陈蔚如,中国药理学报,1990年,11卷,173页

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