甲硫氨酸脑啡肽对抗体和白细胞介素－2的产生和基因转录的影响

Effects of methionine-enkephalin on antibody and interleukin 2production and gene transcription

甲硫氨酸脑啡肽对抗体的产生和Ｔ细胞免疫功能具有调节作用。为了明确这种作用机制，本实验观察了抗体和白细胞介素－２（ＩＬ－２）的产生和基因表达。甲硫氨酸脑啡肽对３Ｆ３杂交瘤细胞的抗体产生及其轻链和重链基因表达影响不明显。较高浓度的甲硫氨酸脑啡肽（０．１ｎｍｏｌ·Ｌ￣（－１）－１μｍｏｌ·Ｌ（－１））不仅促进ＩＬ－２的产生和ＩＬ－２ｍＲＮＡ的转录，而且能提高ＩＬ－２ｍＲＮＡ的稳定性。

Various concentrations (10 fmol . Li-1μmol . L-1) of methionine-enkephalin (met-enk) did notchange the antibody production and genc transcription ofantibody light chain and heavy chain from 3F3 hybridomacells. However, at the higher concentrations (0.1 nmol . L-1 -1μmol . L-1) met-enk not only enhanced IL-2 production andaugmented IL-2 mRNA transcription but also increased thestability of IL-2 mRNA.