摘要
应用抗增生细胞核抗原（ＰＣＮＡ）单克隆抗体ＰＣ１０）对肺癌（ｎ＝８６）和肺炎性假瘤（ｎ＝１０），采用ＳＰ法进行免疫组化染色，计数４００个细胞中的阳性细胞，计算每例的细胞增殖指数（ＰＩ）。阳性细胞数＞５％为阳性。肺癌的阳性率为８３．７２％，而炎性假瘤全部为阴性。细支气管肺泡癌、腺泡状腺癌和大细胞肺癌的ＰＣＮＡ的ＰＩ指数分别为０．２１５、０．３６和０．６６７．Ｎ＿０与Ｎ＿２、Ｍ＿０与Ｍ＿１比较，Ｎ＿２和Ｍ＿１的ＰＩ指数明显高于Ｎ＿０和Ｍ＿０。４６例患者中，术后存活５年以上者的ＰＩ指数明显小于３年以内者Ｐ＜０．０１。认为ＰＣＮＡ是一个有效的肺癌预后的生物标记物。
he expression of PCNA in 86 cases of
non-small cell lung cancer and 10 cases of inflammatorypseudotumor
was studied by using an immunostaining of monoclonal antibody(PC-10
).The positivecells in 400 carcinoma cells and proliferating index PI
were counted in each case. More than 5%PCNApositive cells was
designated PCNA(+).The positive rate was in 83.72%of lung cancers
then all ofthe inflammatory pseudotumors was negative. PI of the
bronchiolo-alveolar ,acinar and large cell carci-nomas were in 0.125,
0.35 and 0.667 respectively.PI was decreased with increasing degree
of histologicdifferentiation. The correlations were observed between
PI and TNM status,the more high PI was themore easily N and M were
metastasized. Survival analysis showed that PI of over 5-year
patients wassignificantly lower than within 3-year patients. The
results suggest that immunostaining of PCNA isuseful for diagnosis
and prognosis of non-small lung cancer.
出处
《中国医科大学学报》
CAS
CSCD
1995年第2期128-131,共4页
Journal of China Medical University
基金
8.5攻关项目
