LAK和TIL细胞的表型测定

Determination of Epitope of LAK and TIL cells

应用多种抗人淋巴细胞的单克隆抗体和流式细胞仪，分析用ＩＬ－２诱生患者淋巴细胞２周形成的ＬＡＫ和ＴＩＬ细胞的表型。结果：ＬＡＫ细胞中ＣＤ＿３￣＋细胞为（８５．０±２．５３）％，ＣＤ＿（１６）细胞为（８．３±１．０）％，证实ＬＡＫ细胞是以Ｔ细胞为主，包括有ＮＫ细胞的混合群体。双标记表型分析ＣＤ＿８￣＋Ｌｅｕ７￣－的ＣＴＬ细胞占８１．４８％，因而推测ＬＡＫ细细胞中，抗原特异性和ＭＨＣ限制性的ＣＴＬ细胞占有很大比例。ＴＩＬ细胞中ＣＤ＿３￣＋细胞为（６４．７±９．７）％，ＣＤ＿（１６）￣＋细胞为（１．６±０．２）％，Ｌｅｕ７￣＋细胞为（６．８±１．１）％。双标记表型分析ＣＤ＿８￣＋Ｌｅｕ７￣－的ＣＴＬ细胞占ＣＤ＿８￣＋细胞的８６．７６％，证实ＴＩＬ细胞主要是Ｔ细胞，ＮＫ细胞极少。

Manifold monoclonal antibodies of antihuman lymphocytes and flow cytometer were used to ana- lyse the epitope of Lymphokine-activated killer cells(LAK)and tumor infiltrating lympocyte cells(TIL), which had been induced from patient lymphocytes for two weeks. Results: In LAK, 85 percent(±2.53%)were CD_3￣+ cells, and 8.3 percent(±1.O%)CD_(16)￣ +. By double marker analysis of epitopeCD_8Leu 7￣-CTL accounted for 62.1 percent.These proved that lAK cells were mixture of T and NKcells, the former being the main component. In TIL, CD_3￣+0 cells were 64.7 percent(±9.7%),CD_(16)￣+1.69 percent(±0.2%),and Leu 76.8 percent(±1.1%). The analysis also showed that, as induc-ing cells of CTL, CD_4 B_4 cells were 82.01, CD_4￣+ 2H_4￣+ 96.4,and CD_8￣+Leu 7 ￣- CTL cells 86.76 percent.