摘要
观察山豆根碱(Dauricine,Dau)及山豆根苏林碱(Daurisoline,Das)对人血小板聚集和粘附功能的影响,并探讨其作用机制。结果表明,两者均可抑制由二磷酸腺苷、花生四烯酸和胶原引起的血小板聚集,药物浓度与聚集抑制率表现出明显的量效关系。Dau处理的血小板其对二磷酸腺苷、花生四烯酸和胶原诱导聚集抑制的IC_(50)(mmol/L)分别为0.042(n=5),0.028(n=9)及0.046(n=10);Das则分别为0.042(n=5),0.026(n=5)及0.057(n=4)。Das表现出对血小板粘附的明显抑制作用。测定经Dau与Das处理的血小板内cAMP浓度,发现Dau不影响血小板内cAMP水平,而Das可使cAMP水平明显升高。提示,Dau与Das有很强的抗血小板生物活性作用,Das的作用可能与血小板内cAMP升高有关。
ur experiment showed
that dauricine and daurisoline could inhibit platelet aggregation
induced byseveral agonists in vitro.The IC_(50)of dauricine to
platelet aggregation stimulated by ADP,AA and colla-gen was
0.042,0.028 and 0.045 mmol/L,and the IC_(50)of daurisoline to
aggregation induced by themwas 0.042,0.026,and 0.057 mmol/L
respectively.Daurisoline also expressed a strong inhibiting effecton
platelet adhesion.The platelets treated with daurisoline had an
increased cAMP level,which mightbe the reason for antiplatelet
ability of daurisoline.
出处
《中国医科大学学报》
CAS
CSCD
1995年第6期579-581,共3页
Journal of China Medical University