脂质过氧化与体外循环中红细胞损伤关系的实验研究

LIPID PEROXIDATION AND RED BLOOD CELL INJURY DURING EXTRACORPOREAL CIRCULATION

本文通过大体外循环模型（ｎ＝６），研究脂质过氧化对红细胞的损伤作用。体外循环期间血浆和红细胞膜过氧化脂质（Ｐ－ＬＰＯ和Ｅｍ－ＬＰＯ）含量增加，红细胞变形能力下降，血浆游离血红蛋白（ＰＦ－Ｈｂ）浓度升高，ＬＰＯ与红细胞滤过指数（ＩＦ）及ＰＦ－Ｈｂ间呈直线正相关。红细胞超氧化物歧化酶及Ｎａ＋－Ｋ＋－ＡＴＰ酶活力均发生改变。结果表明，脂质过氧化与体外循环造成红细胞损伤有重要关系。

To investigate the damaging effect of lipid peroxides (LPO) on red blood cells (RBC) during extracorporeal circulation, six dogs were subjected to cardiopulmonary bypass (CPB) with membrane oxygenator for 120 minutes. During CPB, P-LPO and Em-LPO increased significantly. The damaging effect of LPO on RBC was studied by the measurements of the concentration of plasm free hemoglobin (PF-HB), the filtration index (IF), and Na+-K+-ATPase activity of RBC.Statistical analysis revealed a positive correlation between lipid peroxides and damage of RBC. It is concluded that lipid peroxidation is an important factor of RBC injury during CPB, and the mechanism was briefly discussed.