人冠状动脉粥样硬化中巨噬细胞清道夫受体的免疫组织化学观察

AN IMMUNOHISTOCHEMICAL STUDY ON HUMAN MACROPHAGE SCAVENGER RECEPTORS IN CORONARY ATHEROSCLEROSIS OF 19 AUTOPSY CASES

关于在人冠状动脉粥样硬化病变中巨噬细胞清道夫受体（简称ｈＳＲ）的病理学研究，尚未见文献报道。本文材料选自连续１９例日本人尸俭，其中男１２例，女１７例，４１～８８岁（平均６３．６岁）。在常规尸检中对冠状动脉纵行剖查，取不同阶段的粥样硬化病变，ＰＬＰ液固定，冰冻切片，在阻断内源过氧化酶（Ｉｓｏｂｅ氏法）后免疫酶标染色：第一抗体分别用ｈＳＲＩ－１，２，３；ｈＳＲＩＩ－１（日本），及抗ＥＢＭ１１、ｍｙｏｓｉｎ、ｄｅｓｍｉｎ、ａｃｔｉｎ抗体，ＤＡＢ发色，镜检。结果发现，在人冠状动脉粥样硬化病变中，巨噬细胞（泡沫细胞）内存在两型（ｈＳＲＩ、ＩＩ）受体。在脂纹性病变中，抗ｈＳＲ（Ｉ、ＩＩ型抗体）染色反应强阳性，而在斑块坏死区及纤维斑块中呈弱阳性。说明在重度或陈旧性斑块中ｈＳＲ作用减弱。本研究及文献提示，ｈＳＲ在动脉粥样硬化的发生发展中起重要作用。

The expression and distribution of human scavenger receptors (hSR) in coronary atherosclerosis of human have not been reported. In this study we reported an immunohistochemical observation on hSR in coronary atherosclerosis of 19 Japanese autopsy cases.Arterial specimens were taken from different coronary atherosctlerotic lesions. Immunoenzymatic labelled staining was performed with rabbit anti-hSR antisera:hSR I-1. hSR I-2. hSR I-3 and hSR II-1 , anti-EBM11,myosin, desmin and actin using DAB as substrate. Allsections were observed by light micrascope.It was found that in the foam cells of fatty streaks there was much more intense immuno-reactivity of two types of hSR than those of advanced atherosclerotic plaques indicating the role of hSR decreased in advanced atherasclerosis.This study supports the suggestion that scavenger receptors may play an important role in the development of atherasclerosis.