小蔓长春花组织培养的研究

Studies on Tissue Culture of Vinca minor L.

在含有和不含有水解乳白蛋白的８种诱导培养基中，以ＭＳ培养基添加水解乳白蛋白２ｇ／Ｌ和萘乙酸１ｍｇ／Ｌ诱导小蔓长春花愈伤组织为佳，并且以外植体叶的愈伤组织诱导率为最高，达到９０％。在含不同浓度萘乙酸的８种继代培养基中，以Ｂ５ａ培养基继代培养为好，增长倍数最高可达１４．４３。并经定性试验证明在不同继代培养基上生长的愈伤组织内均含有长春胺。

Studies on callus induction and subculture of Vinca minor L.were reported.In the experiments of callus induction four media contained lactoalbumin hydrolysate were compared with other four media contained no lactoalbumin hydrolysate.It was showed that MS medium supplemented 2 g/L of lactoalbumin hydrolysate and 1 mg/L of naphthylacetic acid was the best,and the induction rate of callus on leaf of explant highly approached 90%.For culture of eight subculture media supplemented different concentrations of naphthylaceticacid were compared.It was showed that the B5a medium was the best, and the amount of cell growth approached 14.43 times.It was also demonstrated that vincamine existed in eallus on all of subcultures by quantitative method.