双波长薄层扫描法测定风油精中华蟾毒精的含量

Determination of the Quantity of Venenum bufonis in Feng You Jing by TLC Dual Wavelength Scanning Method

应用双波长薄层扫描法测定了风油精中蟾酥的含量。特别对蟾酥中含量较高的华蟾毒精进行了定量测定。薄层色谱条件：固定相为硅胶ＧＦ２５４，展开剂为苯－丙酮（７：３）；λｓ＝２８０ｎｍ，λＲ＝３３５ｎｍ；回收率为１００．６％；ＲＳＤ为３．９％（ｎ＝５）。本法准确简便，也可供其它含蟾酥制剂进行定量分析。

The quantity of Venenum bufonis in Feng You Jing is determined by TLC dual wavelength scanning method.This paper only deals with the quantitative determination of cinobufagin──the main active ingredient of Venenum bufonis.The experimental conditions of TLC are as follows:silica gel-G plates with benzene-acetone(7:3V/V),determined by using Cs-9000 dual wavelength TLC scanner at λs=280 nm,λR=335 nm.Recovery is 100.6%,and RSD=3.9%(n=5). This method is accurate and convenient,and has been applied for the quality control of the Feng You Jing preparations.It can be also used as a reference for qualitative and quantitative analysis of other preparations containing Venenum bufonis.