在抗咯萘啶伯氏疟原虫ANKA株中检测抗氯喹伯氏疟原虫ANKA株过度表达的54-kDa蛋白(英文)

Detection of 54-kDa protein overexpressed by chloroquine-resistant Plasmodium berghei ANKA strain in pyronaridine-resistant P berghei ANKA strain

目的:我们曾报道了抗氯喹伯氏疟原虫过度表达54-kDa蛋白,本研究旨在探讨抗咯萘啶伯氏疟原虫是否也过度表达这种蛋白.方法:使用免疫印迹分析及免疫电子显微镜检查.结果:抗咯萘啶疟原虫和抗氯喹疟原虫一样主要过度表达37(36—38)kDa和16(15—17)kDa两条带;在7次实验中有3次检测到54(52—62)kDa和96(95—100)kDa条带.这些蛋白主要散在分布于红内期疟原虫滋养体、裂殖体及裂殖子的胞质中,疟原虫寄生的红细胞中也有少量分布.结论:抗咯萘啶疟原虫和抗氯喹疟原虫都过度表达同一类蛋白质.

AIM:A 54-kDa protein overexpressed by chloroquine-resistant ( CR ) Plasmodium berghei ANKA strain was first reported by us. This study is conducted to detect the protein in pyronaridine-resistant (PR)P berghei ANKA strain. METHODS:Im-munoblotting analysis and immunoelectron microscopy were used. RESULTS: PR parasites, like CR parasites, mainly overexpressed 2 major bands of 37 (36 - 38) kDa and 16 (15 - 17) kDa which were considered to be 2 subunits of 54 (52 - 62) kDa protein. Three of 7 times of experiments showed a 54-kDa and a 96 (95-100) kDa bands. The proteins were localized to be mainly scattered in cytoplasm of trophozoites, schizonts, and merozoites of erythrocytic stage of P berghei, Some of them were distributed in cytoplasm of erythrocytes infected with parasites. CONCLUSION: Both PR and CR parasites overexpressed the same proteins.