松果体和褪黑激素对大鼠脾淋巴细胞增殖和地诺前列酮产生的影响(英文)

Effects of pineal body and melatonin on lymphocyte proliferation and dinoprostone production in rat spleen

目的:研究松果体和褪黑激素对大鼠脾淋巴细胞增殖及地诺前列酮产生的影响.方法:采用松果体切除术,淋巴细胞增殖反应测定及地诺前列酮放射免疫检定法.结果:大鼠脾淋巴细胞增殖反应存在昼夜节律.松果体切除后,此昼夜节律消失,脾淋巴细胞增殖反应降低,而脾脏地诺前列酮增加.16:00ip褪黑激素(Mel)10 μg·kg^(-1)·d^(-1)连续7 d能恢复之,且促进正常大鼠脾淋巴细胞增殖反应,抑制脾地诺前列酮的产生.结论:松果体Mel促进大鼠脾淋巴细胞增殖反应与抑制脾脏地诺前列酮的产生有关系。

AIM:To determine the effects of pineal body and melatonin (Mel) on lymphocyte proliferation and dinoprostone production in rat spleen. METHODS: Pinealectomy ( PE ) , lymphocyte proliferation assay and dinoprostone radioimmunoassay were used. RESULTS: A circadian rhythm of splenic lymphocyte proliferation which peaked at 22: 00 was obliterated by PE in rats. PE led to an impairment of lymphocyte proliferation and an increase of dinoprostone production, which were restored by ip Mel 10 ug. kg-1. d-1 at 16: 00 for 7 d . Mel promoted lymphocyte proliferation and inhibited dinoprostone production in intact rats. A negative correlation between the change in lymphocyte proliferation and dinoprostone production was seen. CONCLUSION. The pineal body and its main hormone Mel play a regulatory role in circadian lymphocyte proliferation which is related to dinoprostone production in rat spleen.