双香豆素对癌细胞的光敏反应及二乙基二硫代氨基甲酸钠的增敏作用

Photosensitization of dicoumarol on tumor cells and enhancement by sodium diethyldithiocarbamate

目的:研究双香豆素(Dic)对肿瘤细胞的光敏反应及二乙基二硫代氨基甲酸钠的增敏作用.结果:Dic 40,80,120 μmol·L^(-1)光敏反应使腹水型肝癌细胞(Hep A)死亡率增加(50％—70.4％),照光组始终高于避光组30％左右.照光组细胞DNA合成抑制率(81％—93％)明显高于避光组(19％—53％),且有浓度依赖性.避光组Dic对细胞也有轻微的损伤.结论:Dic光敏反应对Hep A细胞有较强抗肿瘤活性,DDC能增强Dic对细胞的光敏杀伤作用.Dic—DDC照光组细胞死亡率及DNA合成的抑制率比单独的Dic照光组明显增高.Dic光敏反应与~1O_2和OH无关,而与H_2O_2与O_2密切相关.

AIM: To study the photosensitization of di-coumarol (Dic) on tumor cells and any effect by sodium diethyldithiocarbamate (DDC). RESULTS: Dic,40, 80, 120umol.L-1 con-centration-dependently inhibited the DNA synthesis (81%-93%) and increased the mortality (50%-70.4%) of ascitic hep-atoma (Hep A) cells exposed in light. These 2 indices were changed slightly by Dic alone even in non-illuminating group. DDC enhanced the photosensitization of Dic group. CONCLUSION: Photosensitization of Dic showed strong antitumor activities against Hep A cells. The mortality of Hep A and inhi-bition of the DNA synthesis in Dic-DDC-light group significantly stronger than in Dic-light group. Photosensitization of Dic was not due to 1O2 and OH, but closely related to O2- and H2O2.