精氨酸加压素C-端五肽片段ZNC(C)PR在大鼠脑内的结合位点(英文)

Binding sites of ZNC(C)PR, a pentapeptide fragment of argipressin,in rat brain

目的:研究精氨酸加压素C-端五肽片段ZNC(C)PR在大鼠脑内的结合位点.方法:用[^(35)S]ZNC(C)PR和放射配位体测定法;结果:发现大鼠脑内存在ZNC(C)PR的独特结合位点,以饱和性、可逆性、特异性和高亲和方式与ZNC(C)PR结合,其K_d值为1.69±0.16 nmol·L^(-1),此结合位点在脑内分布较广,以杏仁区和大脑皮层等部位含量较高,而在小脑很低. 结论:这一结合位点可能代表一新受体,并介导ZNC(C)PR的记忆促进作用.

AIM: To study the binding sites of ZNC (C ) PR, a C-terminal pentapeptide fragment of argipressin, in rat brain. METHODS: Using radioligand assay and radioligand [35 S]ZNC (C) PR. RESULTS: It was found that there exist native binding sites bound ZNC(C)PR with high affinity in a saturable, reversible and specific manner. Scatchard and kinetic analyses showed that these sites were homogeneous with a Kd value of 1. 69+0. 16 nmol.L-1(in the presence of MgCl2 10 mmol . L-1 ). The binding of ZNC(C)PR to the sites was at a higher level in the brain regions (such as amygdala, cortex,) and was affected by Mg2+. CONCLUSION: These binding sites represented a new type of receptors and mediated the action of ZNC(C)PR on memory.