中脑导水管周围灰质微量注射吗啡的免疫调节作用(英文)

Immunomodulating effects of morphine microinjected into periaqueductal gray

目的:研究吗啡经大鼠中脑导水管周围灰质对免疫系统的影响.方法;大鼠PAG埋植铜管,经此管微量注射吗啡后观察大鼠细胞因子IL-2,IL-6,TNF及NK活力的变化.结果:PAG微量注射吗啡(0,5 μL,3672 ng)显著促进IL-2,TNF-β的产生及T细胞的增殖,对NK细胞的杀伤活性却有抑制作用,这些促进或抑制作用都能被PAG预先微量注射纳洛酮所阻断,对大鼠脾脏巨噬细胞的IL-6及TNF-α的产生都无影响.结论:吗啡通过PAG的阿片受体影响免疫功能,并且对不同的免疫活性细胞的影响各不相同.

AIM:To study the effects of morphine on immune system through rat brain periaqueduc-tal gray (PAG). METHODS: Three hours after microinjection of morphine through the implanted steel tubes to PAG. splenic cyto-kines interleukin 2 (IL-2), interleukin 6 (IL-6), tumor necrosis factor (TNF), and natural killer cells (NK) activity were measured. RESULTS: Microinjection of morphine (0. 5 uL 3672 ng) into PAG region had no influence on IL-6 and TNF-a (production of splenic macrophages, suppressed the natural killer cell (NK) activity and enhanced T-lym-phocyte functions, including concanavalin A (Con A )-induced T-cell proliferation, IL-2 and TNF-β production. Both the suppressive and stimulating actions were blocked by PAG preinjection of the μ opioid receptor antagonist naloxone (0. 5μL, 1μg), which alone showed the contrary effect to morphine. CONCLUSION: Morphine affected immunofunctions through opioid receptors in PAG, and the influences on various immunocompetent cells were different.