硫酸甘糖酯对兔实验性腹主动脉血栓形成的影响(英文)

Effects of propylene glycol mannate sulfate on thrombosis in abdominal aorta in rabbits

目的:研究硫酸甘糖酯(PGMS)对兔实验性腹主动脉血栓形成的影响.方法:用电粒子计数器测血小板浓度,Clinigamma-1272测放射活性.结果:PGMS抑制凝血酶诱导的兔洗涤血小板聚集的IC_(50)为8.91 mg·L^(-1);其75 mg·kg^(-1)iv后10,30,60 min,血小板粘附抑制率分别为90.4％,41.8％和26.3％,对凝血酶诱导的血小板聚集抑制率分别为99％,122％及110％.用气囊损伤兔腹主动脉内膜后1.5h,每克干重动脉段放射活性,铟-血小板数与生理盐水对照组比较具有明显差异.结论:PGMS抑制受伤兔腹主动脉血小板粘附及聚集.

AIM:To study the effects of propylene glycol mannate sulfate (PGMS) on platelet adhesion, aggregation and thrombosis in abdominal sorta in rabbits. METHODS: Platelet adhesion assay was performed with a platelet-adhesion-meter. The platelet concentration was determined with an electronic particle counter, and the total radio-activity was determined wiht Clinigamma-1272. RESULTS: PGMS inhibited washed platelet aggregation induced by thrombin in vitro. The value of IC50 was 0.9 mg·L-1. (95 % confidence limit = 4. 1-13.7 mg·L-1). At 10, 30, and 60 min after iv PGMS 75 mg·kg-1, the inhibition rates of platelet adhesion were 90. 4 %, 41. 8 %, and 26. 3 % and the inhibition rates of platelet aggregation induced by thrombinwere 99 % , 122 % , and 110 % , respectively. It did not exhibit any noticeable effect on platelet aggregation induced by ADP and collagen at this dosage. After 1. 5 h of iv PGMS 75 mg . kg-1 and total autologous 111 In-platelets 3. 3×109 the radioactivity and dry weight of the injured and uninjured segments were determined. The PGMS group showed a significantly lower radioactivity (1.1±0. 6 MBq), 111 In-platelets (8-3±s 3. 3 ×108) as welll as the radioactivity deposited.g-1 out of the total radioactivity infused % (0. 24+ 0. 21) compared with the control group (3. 7 ±0. 5 MBq, 24. 6 ±3. 5 ×108, and 0. 86±0.25, respectively). CONCLUSION:PGMS prevented the platelet adhesion and aggregation at the injured arterial wall.