粉防己碱对兔血小板聚集和血小板活化因子生成的影响(英文)

Effects of tetrandrine on rabbit platelet aggregation and platelet activating factor generation

目的:探讨粉防己碱(Tet)对兔血小板聚集和PAF生成的影响.方法:卡西霉素(Cal)和PAF诱导血小板聚集的聚集率和Tet对血小板聚集的抑制率被测定;给予或未给予Tet处理之血小板用Cal刺激释放PAF的量也被测定.结果:在4—64 μmol·L^(-1)浓度范围,Tet明显抑制Cal和PAF诱导的血小板聚集.IC_(50)值分别为8.6μmol·L^(-1)和14.0μmol·L^(-1).Tet也浓度依赖性的抑制Cal诱导血小板释放PAF,IC_(50)值为21.0μmol·L^(-1).结论:Tet抑制血小板聚集作用与抑制内源性PAF生成有关.

AIM: To study the effects of tetrandrine (Tet) on platelet aggregation and platelet activating factor (PAF) generation in rabbit platelet-rich plasma (PRP). METHODS: The aggregation rate of platelets induced by calcimycin (Cal) and PAF and the inhibition rate of Tet on platelet aggregation were measured. The amount of PAF in PRP stimulated with Cal and treated with Tet was also measured. RESULTS: Cal 1 - 8μmol · L-1 and PAF 9. 5 - 190. 5 pmol·L-1 induced platelet aggregation. At the final concentrations of 4 -64umol.L-1, Tet inhibited the aggregationinduced by Cal 4 umol·L-1 and PAF 142. 9 pmol·L^1. The IC50(95 % confidence limits) were 8. 6 (6. 0-12. 2) μmol·L-1 for Cal and 14. 0 (6. 4-30. 4) μmol.L-1 for PAF. In the PRP aggregation by Cal, there was a marked increase in PAF content. Tet dependented the release of PAF from platelets by Cal in a concentration-dependent manner, with IC50 of 21 (8-54)μmol.L-1. CONCLUSION: The inhibition effect of Tet on platelet aggregation might be concerned with the reduction of endogenous PAF generation.