多巴胺对小鼠神经母细胞瘤和大鼠神经胶质瘤的杂交(NG108)细胞在体外的毒性(英文)

Toxicity of dopamine to mouse neuroblastoma × rat glioma hybrid (NG108) cells in vitro

目的:研究多巴胺(DA)对小鼠神经母细胞瘤和大鼠神经胶质瘤的杂交细胞NG108的毒性.方法:用MTT法测定NG108细胞的活性.结果:当DA浓度在100 μmol L^(-1)时对NG108细胞有毒性作用.这时的细胞活性仅为对照的1/4左右.DA受体拮抗剂舒必利和Sch-23390不能阻断DA毒性,表明DA对NG108细胞的毒性作用不是由DA受体介导的.DA(125 μmol L^(-1))的毒性作用能被过氧化氢酶(50 kU L^(-1))、超氧化物歧化酶(50kU L^(-1))和维生素C(200 μmol L^(-1))抑制.它们分别能使NG108细胞的活性由DA单独作用时的25.9±11.0％上升到74.8±4.4％、72.3±4.5％和71.4±2.3％.结论:DA对NG108细胞的毒性作用是由DA氧化代谢产生的有毒产物如过氧化氢引起的.

To study toxicity of dopamine to mouse neuroblastoma X rat glioma hybrid (NG108) cells. METHODS: Cell viability was estimated using 3-[4, 5-dimethylthiazol-2-yl ]-2, 5-diphenyltetrazolium bromide (MTT) assay. RESULTS: Dopamine at 100umol L-1 was toxic when added to cultures 24 h after plating. The cell viability was about 1/4 of control. Toxicity did not seem to be mediated by dopaminergic receptors because the dopamin-ergic antagonists sulpiride and Sch-23390 did not block the toxic effect of dopamine. Cata-lase 50 kU L-1, superoxide dismutase 50 kU L-1 and l-ascorbic acid 200 umol L-1 blocked the dopamine (125 umol L-1) toxicity and elevated the cell viability from 25.9±11. 0 % to 74. 8±4.4 %, 72.3±4.5 % and 71.4±2.3%,respectively. CONCLUSION: Dopamine toxicity to NG108 cells was mainly attributed to the oxidation of dopamine and its toxic by-products, eg, H2O2.