吗啡及纳洛酮对淋巴细胞体外增殖的作用(英文)

Effects of morphine and naloxone on proliferation of lymphocytes in vitro

目的:研究吗啡对不同淋巴细胞增殖的作用及纳洛酮的影响.方法:观察吗啡对未成熟的、静止的及活化的脾脏淋巴细胞体外增殖影响及纳洛酮的阻断作用.结果:吗啡(1×10^(-10)—1×10^(-6)mol L^(-1))能增加Con A诱导的T-细胞的增殖,1 μmol L^(-1)还能促进LPS诱导的B-细胞的增殖,同时这些增强作用都能被纳洛酮50μmol L^(-1)阻断,纳洛酮单独亦能促进活化T-细胞的增殖.而吗啡1×10^(-10)—1×10^(-5)mol L^(-1)对静止的脾脏淋巴细胞及Con A活化的胸腺淋巴细胞的增殖都无影响.但是吗啡1mmol L^(-1)能广泛抑制静止的、LPS活化的脾脏细胞及Con A活化的胸腺,脾脏淋巴细胞增殖,且都不能被纳洛酮阻断.结论:吗啡对活化T-和B-细胞的促进作用是由细胞表面的阿片受体介导的,此阿片受体随着淋巴细胞的成熟和活化而变化,而吗啡1 mmol L^(-1)对淋巴细胞增殖的抑制作用却不是由经典的阿片受体介导的.

To study the effects of morphine on different lymphocytes and the influence of halox-one. METHODS:The proliferation rates of unmature, resting, and activated T-lympho-cytes and B-lymphocytes were determined under various concentrations of morphine with or without naloxone in vitro. RESULTS: Morphine 1 ×10~10 - 1×10-6 mol L-1 enhanced concanavalin A (Con A)-induced splenic T-cell proliferation, and 1μmol L-1 enhanced lipopolysaccharide (LPS) -induced splenic B-cell proliferation. Naloxone, which per se enhanced the T-cell proliferation, blocked the enhancing effects of morphine. Morphine (1 ×10-10 -1×10-5 mol L-1) had no influence0 on the proliferation of resting splenocytes and Con A-induced thymus cells. Morphine 1 mmol L-1 inhibited the proliferation of resting , LPS-induced splenocytes, and Con A-induced splenic and thymus cells. These inhibiting effects were not blocked by naloxone (50μmol L-1). CONCLUSION: Stimulating effect of morphine on activated T- and B-cells were mediated by opioid receptors and different opioid receptors existed during the differentiation and activation of lymphocytes. The inhibitory effects of morphine (1 mmol L-1) were not mediated by opioid receptors.