摘要
目的:探讨绞股蓝总皂甙对淋巴细胞转化和NDA多聚酶Ⅱ活性的影响. 方法:采用C_(67)BL/6J和BALB/c小鼠淋巴细胞混合培养及淋巴细胞转化实验,按[~3H]TdR和TTP掺入法检测DNA合成和DNA多聚酶Ⅱ活性. 结果:小剂量Gyp(2.5—20 mg L^(-1))促进淋巴细胞转化,并提高DNA多聚酶Ⅱ活性,促进淋巴细胞DNA合成,而大剂量Gyp(>40 mgL^(-1))则呈相反作用. 结论:Gyp能通过调节DNA多聚酶Ⅱ活性调控淋巴细胞转化.
To study the effects of gypenosides (Gyp ) on lymphocyte transformation and DNA polymerase Ⅱ activity. METHODS: Lymphocyte transformation response was induced by concanavalin A and lipopolysac-charides respectively. The activity of DNA polymerase Ⅱ and DNA synthesis were assayed with TTP and [3H]TdR incorporation respectively in mixed lymphocyte culture test. RESULTS:Gyp 2.5 - 20 mg L^1 enhanced splenic T- and B- cell transformation, increased the DNA synthesis and potentiated the activity of DNA polymerase Ⅱ . Howerer, Gyp > 40 mg L-1 showed contrary effects. CONCLUSION: Gyp regulated lymphocyte transformation and DNA synthesis by regulating DNA polymerase Ⅱ activity.
出处
《中国药理学报》
CSCD
1995年第4期322-324,共3页
Acta Pharmacologica Sinica
关键词
绞股蓝
皂甙类
淋巴细胞转化
小鼠
Gynostermma pentaphyllum
saponins
DNA polymerase II
lymphocyte transformation
mixed lymphocyte culture test
