番泻甙和大黄多糖对大鼠血小板细胞内游离钙浓度的影响

Effects of sennoside and rhubarb polysaccharides on the cytoplasmic free calcium in the platelets of rats

采用钙荧光探针Ｆｕｒａ－２定量分析的方法研究了大黄有效成分番泻甙和大黄多糖对血小板细胞内游离钙浓度的影响。结果表明，血小板细胞在静息状态下胞浆内［Ｃａ２＋］ｉ为１４２．０９±１７．６９ｎｍｏｌ．Ｌ－１，当依次加入ＣａＣｌ２和凝血酶后，血小板细胞内游离钙浓度［Ｃａ２＋］ｉ均明显高于静息时的水平（Ｐ＜０．０１）。预先向血小板悬液中加入番泻甙８×１０－５～４×１０－１ｇ或大黄多糖１×１０－１ｇ后，再依次加入ＣａＣｌ２和凝血酶，此时血小板细胞内游离钙浓度较对照组明显降低（Ｐ＜０．０１），其抑制效应与剂量相关。提示大黄及大黄制剂抑制血小板细胞聚集与番泻甙和大黄多糖抑制钙内流有关。

The effects of sennoside and RPP (Polysaccharide from rheum palmatum) on the cytoplasmic free calcium in the platelets of rats were studied by means of fluorescent probe (Fura-2) quantitative The project supported by National Natureffeence Foundation of Chiinaanalysis. The resting free Ca2+ level was 142.9±17.69 nmol·L-1 in the platelets,After adminstration of CaCl2 (2 mmol·L-1)and thrombin (500 U·L-1) sequentially.the free Ca2+ levels obviously elevated (P<0. 01),compared with the resting level ,After administration of sennoside and RPP previously. the free Ca2+ levels significantlydreased (P<0.01).conpared with thatof the control grop.Furthermore. the effects of drugs were dose-dependent.Therefore. it suggeststhat the effect of rhubarb and rhubarb preparation on inhibiting aggregation of platelets is related to sennoside and RPP.which can inhibit influx of Ca2+.