维拉帕米对人血PMN细胞呼吸暴发和氧自由基的抑制作用

Inhibitory effects of verapamil on respiratory burst of polymorphonuclear leucocytes

用化学发光法测定：人血ＰＭＮ细胞受ＰＭＡ刺激发生呼吸暴发产生的活性氧；黄嘌呤－黄嘌呤氧化酶体系产生的Ｏ；Ｖｉｔｃ－ＣＵ２＋－酵母多糖产生的·ＯＨ及Ｈ２Ｏ２的释放，同时也观察了维拉帕米对上述体系产生的自由基的影响。结果提示，维拉帕米对ＰＭＮ细胞的呼吸暴发有显著的抑制作用；对Ｏ、Ｈ２Ｏ２有清除作用，呈剂量依赖性，其ＩＣ５０分别为２８．１，２２．３ｎｍｏｌ·Ｌ－１；对·ＯＨ则无作用。

Chemiluminescence method was used to measure the effect of verapamil on (a) active oxygen species generation in respiratory burst of human blood polymorphonuclear leucocytes (PMNs) stimulated with phorbol myristate acetate (PMA), (b) superoxide anion (O) production in xanthine-xanthine oxidase system, (c) hydroxyl radicals(·OH) generated by Vit C-Cu2+-zymosan, and(d)the release of hydrogen peroxide ( H2O2). The results showed that verapamil had inhibotory effects on respiratory burst of PMNs, O production and H2O2 release in dose-dependent manner. The IC50 for O and H2O2 was 28.1 and 22.3 nmol·L-1, respectively. No effect of verapamil on·OH production was measured.