金丝桃甙对心肌脂质过氧化的影响

Effect of hyperin on myocardial lipid peroxidation

用ＴＢＡ法测定丙二醛（ＭＤＡ）含量，观察金丝桃甙（Ｈｙｐ）对阿霉素以及缺氧／再灌注所致心肌脂质过氧化的影响。结果表明：Ｈｙｇ１００ｍｇ·ｋｇ－１和ＥＧＴＡ４００μｍｏｌ·ｋｇ－１ｉｐ可明显抑制阿霉素所致小鼠心肌ＭＤＡ含量的升高，ＨＰＰ可增强ＥＧＴＡ的作用，Ａ２３１８７拮抗Ｈｙｐ的作用。ＨｙＰ１０、１００μｍｏｌ·Ｌ－１可显著减少缺氧；再灌注离体大鼠心脏ＭＤＡ的产生。提示Ｈｙｐ具有抗心肌脂质过氧化的作用，此作用可能与其阻滞钙内流有关。

The effect of hyperin(Hyp) on adriamycin-and hypoxia/ reperfusion-induced myocardial lipid peroxidation was investigated using malondialdehyde (MDA) as an index. The increase in MDA in murine cardiac tissue after administration of adriamycun (15 mg·kg-1, ip) was significantly inhibited by Hyp 100 mg·kg-1 and EGTA 400 μmol·kg-1 ip. A23187 antagonized the effect of Hyp. Hyp enhanced the effect of EGTA. Also, Hyp 10, 100μmol· L-1 markedly reduced the increase in MDA production in isolated rat hearts after hypoxia/ reperfusion. The results suggest that Hyp possess an antiperoxidative activity, and this effect may be related to blocking calcium influx.(Now in Anhui Tunxi Second Pharmaceutical Factory,Huangshan 245011)