牛磺酸对翻转小鼠离体小肠葡萄糖──钠转运电位的影响

The effect of taurine on transmural potential of mouse small intestinein v itro

利用翻转小鼠离体小肠囊的方法，观察牛磺酸对葡萄糖－钠转运电位（简称ＰＤ）的影响，借以了解牛磺酸对与糖－钠转运电位密切相关的Ｎａ＋·Ｋ＋－ＡＴＰ酶的影响。结果２．５ｍｍｏｌ·Ｌ－１和５ｍｍｏｌ·Ｌ－１的牛磺酸均可提高ＰＤ（Ｐ＜０．０１），而１０ｍｍｏｌ·Ｌ－１和２５ｍｍｏｌ·Ｌ－１的牛磺酸则对ＰＤ产生相反的作用（Ｐ＜０．０１），推测不同浓度范围的牛磺酸可对Ｎａ＋·Ｋ＋－ＡＴＰ酶产生双向调节作用。

The electrical potential difference(PD) was measured across the,wall of reverted small intestinal sacs.The effect of taurine on transmural potential related to Na+ andglucosetransport was investlgated.Showing taurlne(2.5 mmol· L-1 and 5 mmol·L-1) increased the PD( P<0.01) .While its dose was increased to 10～25 mmol· L-1,its opposite effect on PD was observed( P<0.01) .Base on the theory of active trasPort of glucose,we inferred that taurine posslblly produces the two way effeet on Na+· K+-ATPase,which may explain whythe different dose of taurine produces distincteffect on zinc transport in smallintestine.