摘要
用Ｐｅｒｃｏｌｌ密度梯度离心法分离大鼠肾皮质远曲小管细胞（ＤＴＣ）和近曲小管细胞（ＰＴＣ）．应用放射性同位素￣（４５）Ｃａ示踪技术观察川芎嗪对Ｃａ￣（２＋）跨膜转运的影响，并且采用蛋白质竞争结合法检测了胞膜腺苷酸环化酶活性及细胞内环磷酸腺苷（ｃＡＭＰ）含量。结果表明川芎嗪明显抑制ＰＴＣ和ＤＴＣ细胞￣（４５）Ｃａ的摄取，但对￣（４５）Ｃａ的外排无明显影响。川芎嗪对ＰＴＣ和ＯＴＣ胞膜腺苷酸环化酶活性无影响，但使细胞内ｃＡＭＰ含量显著降低。结果提示川芎嗪影响肾小管细胞钙的摄取，其机理可能与其诱导其它物质使细胞内ＣＡＭＰ水平降低有关。
In order to know howtetramethylpyrazine(TMP)acts on renal calcium trans-port,proximal tubular cells(PTC) and distal tubularcells(DTC)were separated by using Percoll's gradientmethod. 45Ca was used to study the influence of TMPon calcium transmembrane. It was found that TMPsignificantly reduced the 45Ca uptake of PTC and DTC,but did not influence the 45Ca effiux. TMP also reducedthe cAMP content in PTC and DTC.but did not influ-ence the activity of adenylcyclase.The results suggestthat TMP takes part in regulating the Ca2+ transportof PTC and DTC and its effect may be related to the re-duction of cAMP content in the cells.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
1995年第4期274-276,共3页
Chinese Journal of Pharmacology and Toxicology
基金
国家自然科学基金
关键词
川芎嗪
钙45
肾小管细胞
腺苷酸环化酶
tetramethylpyrazine
calciumradioisotopes
kidney tubules,proximal
kidney tubules,distal
epithelium
adenyl cyclase,adenosine cyclicmonophosphateSupported bv the National Natural Science Foundationof China.NO 38970833
