银杏叶中银杏内酯的分离与结构测定

Study on the Structure of Ginkgolides in Ginkgo Biloba Leaves

从银杏叶中分离得到银杏内酯Ａ（１），银杏内酯Ｂ（２）和银杏内酯Ｃ（３），其中用氢氢二维谱（１Ｈ－１ＨＣＯＳＹ）、碳氢二维谱（１３Ｃ－１ＨＣＯＳＹ）及远程碳氢二维谱（Ｌｏｎｇ－ｒａｎｇｅ１３Ｃ－１ＨＣＯＳＹ）等方法对银杏内酯Ｃ的氢信号和碳信号进行了较为完满的归属，并首次用ＮＯＥ差谱技术测定了银杏内酯Ｃ的相对立体构型。此外，还报道了银杏内酯Ｂ和Ｃ的抗血小板活化因子（ＰＡＦ）活性。

Three kinds of Ginkgolides were isolated from the leaves of Ginkgo biloba. They were identified, respectively , as Ginkgolide A (1), Ginkgolide B (2) and Ginkgolide C (3). In the identification of Ginkgolide C ,￣1H-￣1H COSY ,￣13C-￣1H COSY and long-range ￣13C-￣1H COSY were applied- Signal assignment of protons and carbons of Ginkgolide C was completely achieved.Further more, difference NOE spectra were used to confirm the relative configuration of Ginkgolide C for the first time. In addition, the anti-PAF activities of Ginkgolide B and C were recognized.