双嘧达莫对鬼臼乙叉甙抗肿瘤作用及药代动力学的影响

Effects of dipyridamole on the cytotoxicity and pharmacokinetics ofetoposide

体外实验发现，双嘧达莫（ＤＰ），１０ｕｇ／ｍｌ单独作用于人白血病细胞（ＨＬ－６０细胞），不能引起活细胞计数明显减少；ＤＰ与鬼臼乙叉甙（ＶＶ－１６）联合作用于ＨＬ－６０细胞，活细胞计数较单用ＶＰ－１６时明显降低。家兔体内实验表明，ＤＰ不能明显改变ＶＰ－１６的药代动力学参数；ＤＰ有可能对ＶＰ－１６的代谢方式有微弱的影响。本实验结果提示，ＤＰ在体内有可能在不明显影响ＶＰ－１６体内过程的条件下增强其抗肿瘤的作用。

In vitro,the effect of dipyridamole(DP )and etoposide(VP-16 )on humanleukemia-60(HL )cells was examined by trypan blue exclusion. It was found that DP had not cy-tocidal effect on the cells ,and there were significantly fewer viable cells in the case of concomitantexposure to DP and VP-16 than in the case of only exposure to VP-16.In vivo,the pharma-cokinetics of VP-16 was studied in rabbits by determining the concentration of VP-16 in serum byHPLC. It was known that DP could not significantly change the pharmacokinetic parameters ofVP-16,and DP had slight effect on the metabolic manners of VP-16.The results indicate that DP,in vivo,might augment the antitumor activity of VP-16 without obvious action on its pharma-cokinetics.