逆转录病毒介导的重组TNF－α抗小鼠肿瘤效应的研究

The Anti-Tumor Activity of Retrovirus-Mediated TNF-αGene Transfer in Murine Tumor Cells

利用逆转录病毒介导的基因转移技术，将重组ＴＮＦ－α基因导入小鼠平滑肌肉瘤细胞系Ｓ１８０中，对转导后肿瘤细胞的增殖、致瘤效应等进行体外和体内的观察。结果表明；逆转录病毒中介的重组ＴＮＦ－α基因导入，在小鼠体外和体内都有显著的抗肿瘤效应，这为ＴＮＦ－α的肿瘤基因治疗的临床应用奠定了基础。

NF-αgene was expressed in murine S180 cells(BALB/c ) by retroviral gene transfer.The trans-ferred S180 cells were inoculated into BALB/c mice s.c. to observe the tumorigenicity in vivo and in vit-ro. In 50 days, all the five mice inoculated with S180 cells developed tumors , while only one out of fivemice inoculated with TNF-αgene transferred S180 cells developed tumor and in it tumor manifestationwas significantly delayed. The results showed that retrovirus-mediated TNF-αgene transfer suppressedtumorigenicity of S180 cells in vivo significantly,which were the basis for its clinical application of genetherapy for cancer.