FISH前Q显带基因定位方法及其应用

A Modified Method for Fluorescence in Situ Hybridization of Q-banded Chromosomes

应用改进的ＦＩＳＨ（ＦｌｕｏｒｅｓｃｅｎｃｅＩｎＳｉｔｕＨｙｂｒｉｄｉｚａｔｉｏｎ，ＦＩＳＨ）前Ｑ显带方法基因定位，获得良好的Ｑ显带核型和ＦＩＳＨ信号，结合共焦激光扫描显微镜ＭＲＣ－６００系统记录杂交信号和染色体图像，经Ｃｏｍｏｓ系统软件处理，提高了信号的分辨率和敏感性，首次将人类内α胰蛋白酶抑制因子２．５ｋｂ小片段ｃＤＮＡ定位于３Ｐ２１。

luorescence in situ hybridlzation(FISH ) was carried out on metaphase and pre-metaphase chro-mosomes treated by a modified Q-banding, The hybridized signal and chromosomal image were recordedby confocal laser scanning microscope(MRC- 600 ) and processed with COMOS software. The resolu-tion and sensitivity of signal were improved.We had successfully applied the modified procedure to lo-calize the inter α trypsin inhibitor gene on chromasome 3p21 using a 2. 5kb cDNA fragment of the gene.