Ha－ras癌基因蛋白在肺腺癌中过表达及对预后意义

Prognostic Significance of the Expression of Ha-ras Oncogene Product in Lung Adenocarcinoma

用Ｈａ－ｒａｓｐ２１单克隆抗体对５６例获得随访结果的肺腺癌及５例大细胞肺癌的石蜡标本，采用ＳＰ免疫组化法和微波技术进行染色。５０例反应阳性，总阳性率为８１．９７％。其中强阳性率为４５．９％，中度阳性率为３６．０６％。细支气管肺泡癌的强阳性率明显高于腺泡状腺癌和大细胞肺癌（Ｐ＜０．０５）。染色强度与肿瘤的分化程度有关，分化程度越高，染色越强。阴性和中度阳性的５年存活率明显高于强阳性者。本文认为，ｐ２１蛋白的过表达，可以做为肺腺癌预后的一个指标。阳性率与ＴＮＭ分期无关。

he expression of Ha-ras P21 was investigated with the SP immunohistochemical method in paraf-fin-embedded specimens of 56 lung adenocarcinoma cases and 5 large cell carcinoma cases with ascer-tained survival duration. The total positive rate was in 81. 97%,moderate and strong reactions were in36.06 and 45.9%respectively. The strong positive rate of bronchiloalveolar carcinomas was higherthan that of acinar adenocarcinomas and large cell carcinomas(P<0. 05).The incidence and grade ofHa-ras P21 expression increased with the degree of histologic differentiation. Survival analysis showedthat patients with P21-negative or moderate positive(+) tumors had significantly longer survival timesthan those with P21 ++tumors(P<0. 01). The results suggested thatenhanced Ha-ras P21 expres-sion may be one of the clinical prognostic markers in such lung adenocarcinomas. The P21 staining in-tensity was not correlated with TNM classification.