白血病细胞中蛋白激酶C底物的研究

Studies on the Substrate of Protein Kinase C in Leukemia Leucocytes

白血病细胞ｉｎｖｉｖｏ蛋白质磷酸化实验结果表明，当ｆＭｅｔ－Ｌｅｕ－Ｐｈｅ三肽或促癌剂ＴＰＡ作用到白血病细胞使ＰＫＣ活化时，活化的ＰＫＣ可特异地促进白血病细胞中包括分子量为４０ＫＤ蛋白质在内的三种蛋白质磷酸化。这三种蛋白质的磷酸化反应，可被抗癌药物硫杂脯氨酸衍生物所抑制。

he results of protein phosphorylation of leukemia leucocytes in vivo indicated that protein kinase C(PKC)was activated when a fMet-Leu-Phe tripeptide or carcinogenic agent TPA was added intoleukemia leucocytes,and the activated PKC can specially promote protein phosphorylation of threekinds of proteins one of them is,which incluted 40KD protein,in leukemia leucocytes. The phosphoryla-tion of these three proteins could be inhibited by an anticarcinogen thioproline derivatives.