人骨髓基质细胞株培养上清对造血干细胞增殖作用的研究

The study of the Proliferative Function or Human Marrow Stromal Cell Lines Culture Supernate to Hematopoietic Stem Cell

应用ＳＶ－４０导入法建立了人骨髓基质细胞株ＳＣ５－７，然后收集其培养上清，测定其中三种造血因子含量，并将这种培养上清添加至造血干细胞的液体培养基内，每周一次取出培养的细胞，进行计数。同时移至甲基纤维素培养基内继续培养，１４日后，在倒置显微镜下观察并计数各种造血干细胞集落形成的数量。结果：１．人骨髓基质细胞株ＳＣ５－７的培养上清中含有较高浓度的Ｇ－ＣＳＦ、ＧＭ－ＣＳＦ和Ｍ－ＣＳＦ。２．添加ＳＣ５－７培养上清的培养系，比没有添加ＳＣ５－７培养上清的培养系造血干细胞体外增殖时间明显延长。结果表明：人骨髓基质细胞株ＳＣ５－７具有分泌造血因子的作用，而且在体外可以促进造血干细胞的增殖。

arrow stromal cell and its secretion of hematopoietic factors play an important role in the prolifer-ation and differentiation of marrow hematopoietic stem cell.To survey this function,we build marrowstromal cell lines SC5-7 by inputting SV- 40, then collect its culture supernate,measure the quantity ofthree hematopoietic factors in it,and put this culture supernate into the liquid culture medium ofhematopoietic stem cell,then take the culture cell to count it once a week,meanwhile remove it to themethylcellulose culture medium to continue to culture. After 14 days,observe and count the colonynumbers of all kinds of hematopoietic stem cell.Results:1.There are higher consistency of G-CSF,GM-CSF and M-CSF in the culture supernate of human marrow stromal cell lines SC5-7.2.The proliferationtime of the hematopoietic stem cell in vitro of the culture medium with SC5-7 culture supernate is evi-dently longer than that of the culture medium without SC5-7.This result suggests that human marrowstromal cell lines SC5-7 has the fonction of secreting hematopoietic factors,and promoting the prolifera-tion of the hematopoietic stem cell in vitro.