摘要
应用套式聚合酶链反应(PCR)检测1~6型嗜肺军团杆菌,两轮PCR都分别出现了996bp和650bp的特异性扩增产物;而金黄色葡萄球菌等七种细菌均未出现物异性扩增。两轮PCR分别可以检测10pg和10fg的模板DNA。8份嗜肺军团杆菌含量分别为1~10 ̄7cfu/ml的痰感染模拟标本均出现了特异性扩增产物。提示该方法可用于临床,特别是检测含微量嗜肺军团杆菌的临床标本。
e amplified the samples of
legionella pneumophila serogroups 1 to 6.The specific
amplificationbands of 996bp in the first step and 650bp in the second
step were observed respectively. Samples ofother 7 bacteria,
including staphylococcus aureus, didn’t generate positive
signals.The first and thesecond step PCR achieved the sensitivity as
small as 10pg and 10fg of the target DNA respectively. Eight sputum
samples which had been seeded with 1~10 ̄7 cfu/ml of legionella
pneumophila were all pos-itive after nested PCR amplification. We
demonstrated that the nested PCR method was superior in sen-sitivity
and specificity for the detection of legionella pneumophila. The
results suggested that thismethod could be applied in clinic,
especially for detecting samples containing minute LP.
出处
《中国医科大学学报》
CAS
CSCD
1995年第6期575-577,共3页
Journal of China Medical University
基金
辽宁省科委