摘要
采用剖腹取胎的5月龄胎儿,分离其胸腺细胞,经过筛选培养,获得能传代培养的细胞株,在体外连续培养已2年多,共传200余代,命名为人胎儿胸腺细胞株8810(简称HFT8810)。该细胞生长繁殖旺盛、迅速,经多次液氮冻存复苏后,细胞仍生长良好。经长期传代培养,该细胞仍保持分泌胸腺素的功能。
Thymocytes were isolated from a five month fetus after caesarean section and cultured in vitro. A cell line named human fetal thymocyte 8810(HFT8810)was established and cultured continuously for 2 years in our lab,and grew well after recovery from the state of being frozen in liquid nitrogen.The HFT 8810 cells retained their abiliy to secrete thymosin after many generations in vitro culture.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1995年第1期52-56,共5页
Acta Academiae Medicinae Sinicae
基金
国家自然科学基金
关键词
胸腺细胞
人胎儿细胞
细胞株建立
thymocyte
human fetal cells
establishment of cell line