人肝癌裸小鼠模型的建立及其生物学特性的研究

Establishment and Biological Characteristics of the Nude Mice Xenograft Model from Human Hepatocellular Carcinoma

将７例原发性肝癌手术切除标本分别移植于ＢＡＬＢ／ｃ裸小鼠皮下，１例获得成功，并能在裸鼠体内连续传代。１１个月内已传了１０代。其潜伏期为１１ｄ左右，荷瘤鼠血清可测得甲胎蛋白（ＡＦＰ）、γ－谷氨酰转肽酶（γ－ＧＴ）和酸性同工铁蛋白（ＡＩＦ）三种有意义的肝癌血清标记物。移植瘤病理结构与瘤原相似，其遗传学特征与人类恶性肿瘤相同。经连续传代后的荷瘤鼠未见肿瘤局部及远处转移。利用移植瘤行体外细胞培养的结果表明，其生长情况明显好于手术切除标本。

The human hepatocellular carcinoma was successfully heterotrasplanted into nude mice and 10 passages were made seriously during 11 months,InitiaI take rate was 14.3%and then the take rate increased to 1 00%after the 3rd passage.Histological,morphological,ultrastructural and biological investigation and chromosome analysis showed that the characteristic of transplanted tumor was identical to that of characteristics of human donor tumor.Through serial passages no metastatic signs were found grossly and under the microscope in the lymph nodes,liver and lung of the nude mice,Result also showed that the model retained some functions of the originaI tumor,The transplanted tumor could secret a-fetoprotein(AFP),r-glufamyl transpeptidase(r-GT).Acidic isoferritin(AIF)also could be detected.The tumor tissue of xenograft model was cultured in vitro in order to develop a permanent cell line.We found that the tumor specimen of the later passages grew more rapidly than the earlier ones,Up to now 3 cell passages were made.In this paper,a reliable animal model of human hepatocellular carcinoma is provided for studies of clinical diagnosis,treatment,antitumor drug screen test,etc.