人绒癌裸鼠移植瘤模型的建立及其放射免疫显像

Construction and Radioimmunodetection of Nude Mouse Xenograft of Human Choriocarcinoma

将人绒癌新鲜组织碎块接种于Ｂａｌｂ／ｃ裸鼠皮下，成功地建立了移植瘤模型，并已传代１６～６８代，荷瘤裸鼠腹水或瘤内血ｈＣＧ达４００～２００００ｍＩＵ／ｍｌ以上。利用１３１Ⅰ标记的小鼠抗人绒毛膜促性腺激素单克隆抗体（抗ｈＣＧＭｃＡｂ）对荷瘤裸鼠进行放射免疫显像，结果标记抗体能够特异性地聚集于肿瘤部位，使肿瘤显像，最佳显像时间为注入标记抗体后７２～９６ｈ。

Fresh tissue of human choriocarcinoma was injected subcutaneously into the BALB/C nude mouse. The xenografts had grown up with l6～68 passages.The hCG level of the ascites or intratumoral blood was 400～20000mIU/ml. Radioimmunodetection was performed using 131I labelled murine anti-hCG McAb. The labelled antibody could be specifically accumulated in the tumor sites and the tumor imaging was acquired. The ideal imaging time was72～96 hours after the injection of antibody.