低压缺氧大鼠肺外动脉胶原分布的变化

Changes of Collagen Distribution in Extrapulmonary Arteries of Hypoxia-induced Pulmonary Hypertensive Rat

观察模拟５０００ｍ连续低压缺氧７ｄ肺动脉高压大鼠肺外动脉形态学的变化及Ⅰ、Ⅲ型胶原纤维分布的变化，并观察川芎嗪、７６４－３两种中药的疗效。结果显示，低压缺氧大鼠肺外动脉以中、外膜增厚为主。苦味酸天狼星红－偏振光法观察表明肺外动脉主干中膜浅黄色、黄色、红色Ⅰ型和绿色Ⅲ型胶原纤维均增多，外膜以Ⅲ型胶原纤维增多最显著。川芎嗪治疗能部分缓解肺动脉主干和肺外分支增厚，并对主干的Ⅰ、Ⅲ型胶原纤维的增加具有一定的抑制作用。７６４－３对肺动脉主干和分支Ⅰ、Ⅲ型胶原的增多均无明显抑制作用。透射电镜证明，肺外动脉中膜平滑肌细胞肥大，周围胶原纤维增多，外膜成纤维细胞增生肥大，分泌活跃。提示缺氧性肺动脉高压后肺外动脉结构重建主要发生在中膜和外膜，以主干外膜Ⅲ型胶原纤维增多为主。

Changes of collagen distribution in extrapulmonary arteries indudced by hypoxia and the effects of ligustrazine(ligus)and 764-3 were investigated. Histological study showed that hypoxia could induce an increase of the medial and adventitial thickness of the pulmonary arterial trunk.. An increase of type I and type Ⅲ collagen fibers in pulmonary arterial trunk,particularly the increase of type Ⅲ collagen fibers in adventitia of the pulmonary trunk were revealed by picrosirius-polarization methods. Ligus partially prevented the increase of typeI and typeⅢ collagen fibers of pulmonary arterial trunk. Electron microscopy showed thatboth the medial smooth muscle and adventitial fibroblasts were hypertrophic and hyperplastic, and the surrouding collagen fibers were increased.