绿脓毒素PE40区段的基因克隆和序列分析

Cloning and Sequencing of the Gene PE40,Coding for a Portion of Pseudomonas Exotoxin A

利用ＰＣＲ技术从绿脓杆菌ＰＡ１０３总ＤＮＡ中扩增出编码不含结合细胞功能区的绿脓毒素区段ＰＥ４０基因，经测序验证后将其克隆至表达载体ｐＢＶ２２０中，该重组质粒可以用于多种分子导向药物的研究。

Gene PE40 coding for a mutant form of Pseudomonas Exotoxin A was devoid of its native cell binding dornain, and was amplified from the total DNA of P. aeruginosa PA103 by PCR. After being sequenced , it was cloned into expressional vector pBV220 to get a recom binant plasmid, which will be useful in the research of molecular targeted protein.