P物质在雷公藤引起免疫抑制中的作用

The Role of Substance P in Immunosuppression Induced by Tripterygium Wilfordii

本实验观察了雷公藤与Ｐ物质（ＳＰ）对免疫功能的影响，并利用辣椒素（ＣＡＰ）耗竭大鼠脊髓后角ＳＰ，进一步探讨了脊髓后角ＳＰ在雷公藤引起免疫抑制中的作用。实验结果提示雷公藤具有抑制细胞免疫的作用，其机制可能与脊髓后角ＳＰ含量增高有关，脊髓蛛网膜下腔注射（ｉｔｈ）辣椒素耗竭脊髓后角ＳＰ，既可提高白细胞介素－２（ＩＬ－２）的产生水平，也可减轻雷公藤所引起的免疫抑制效应，表明脊髓后角ＳＰ可能参与雷公藤的细胞免疫抑制作用。

The effects of T Ⅱ(extracted from Tripterygium wilfordii)and substance P SP)on imunoregulation were investigated.It has been shown that Tripterygium wilfordii is an immunosuppresive.In order to assess the immunosuppression elicited by T Ⅱ was mediated by SP in spinal dorsal horn,Wistar rats were injected intrathecally with capsaicin(CAP)to deplete SP in spinal dorsal horn prior to TⅡgiven orally.The level of interleukin 2(IL-2)produced by splenic lymphocytes of rats and the contents of SP in some brain areas and spinal cord in mice were assayed after T Ⅱ treatment. Our findings were as follows:1.The level of IL-2 was decreased significantly after T Ⅱ treatment only。 However the level of IL-2 was increased markedly after CAP was given intrathecally, Moreover, CAP administration also enhanced the level of IL-2 even in immunosuppression group induced by T Ⅱtreatment.2.SP contents in spinal cord,hypothalamus and brain stem of mice were increased dramatically during immunosuppression produced by T Ⅱ.The results suggested that SP in spinal dorsal horn was involved in regulation of cellular immunity.the suppresive effect of T Ⅱ on cellular immunity might be mediated by SP in spinal dorsal horn.