摘要
本文研究了维生素A和黄芪在逆转烧伤后免疫抑制的作用。用体外培养T淋巴细胞集落形成法,观察脾—T淋巴祖细胞值,和氚—胸腺嘧啶核苷掺入法的淋巴细胞转化试验,反映鼠淋巴细胞对PHA刺激转化的功能,观察到烧伤后接受维生素A治疗,显示免疫抑制有所改善。说明维生素A是逆转烧伤后细胞免疫抑制的有效制剂。
In present study the effects of vitamin A and Astargalus on the splenic TL-CFU of mice were studied by means of T-lymphocytes colony formation in semi-solid culture in vitro and incorporation of ~3H-TdR. Marked reduction of the responsive reaction of TL-CFU and T-lmphocyte transformation were found. The TL-CFU of the experimentally burned mice untreated with vitamin A (i. e. group 1) were significantly inhibited (p<0.005) in comparison with the unburned control group (i. e. group 4). And the TL-CFU of the experimentally burned mice treated with vitamin A (i. e. group 3) increased significantly (p<0.005) in comparison with group 1. Incorporation of 3H-TbR showed that vitamin A might accelerate the proliferation of the TL-CFU of the burned mice. It means that Vitamin A might be regarded as an effective agent for the reversal of the inhibition of cell-mediated immunity in post-burned state,whether Abstragalus plays a role in regulating immune inhibition needs further investigation.
