激光照射、免疫抑制剂及其配合对免疫细胞的影响

Effects of large dose of He-Ne laser,immune inhibitors and their combination on cellular immunity in rats

应用３９．７２Ｊ／ｃｍ￣２的Ｈｅ－Ｎｅ激光连续１４天照射健康大鼠脊背正中线皮肤，并同时分别与亚剂量的免疫抑制剂环孢霉素Ａ、硫唑嘌呤相配合，观察了它们对Ｔ、Ｂ淋巴细胞数、淋巴细胞转化刺激指数（Ｓ、Ｉ）、１０￣６个淋巴细胞ＣＰＭ数的影响。结果表明：大剂量Ｈｅ－Ｎｅ激光和Ａｚ唑嘌呤均可降低大鼠Ｂ淋巴细胞数。大剂量Ｈｅ－Ｎｅ激光、环孢霉素Ａ、硫唑嘌呤及激光照射与二者配合，均可使淋巴细胞转化刺激指数（Ｓ、Ｉ）在试验后４周内较试验前明显降低。

Healthy rats were irradiate by He- Ne laser in the dose of 3 9.7245J/cm￣2 on the midline of dorsal skinfor 14 days continuously and the medication of a immune inhibitor(CsA or Aza)at a lower dose was applied at thesame time.The numbers of T-cell and B-cell,S.I and the number of CPM of 10￣6 lymphocytes were monitored for 4weeks after the above stated treatment were applied.The results are as follow:Both laser irradiation and Aza candecrease the number of B-cell;S.I,was significantly decreased in 4 weeks after the freatment of large dose He-Nelaser irradiation,inedication of immune inhibitor(Aza or CsA)or their combination.