正加速度暴露时兔血清超敏C反应蛋白的变化与心血管损伤风险预测的关系

Relationship of high-sensitivity C-reactive protein changes in rabbit's serum and prediction of cardiovascular risk by repeated and long-term + Gz exposure

目的 探讨重复正加速度(+Gz)暴露时兔血清超敏C反应蛋白(high-sensitivity C-reactive protein,hs-CRP)的变化与心血管损伤风险预测的关系. 方法 将24只动脉粥样硬化实验兔随机分为对照组和+Gz暴露4周、8周、12周组,每组4只.各组兔实验结束后留取静脉血,测定hs-CRP含量,并用电镜观察兔主动脉组织切片. 结果 +Gz暴露对兔静脉血hs-CRP含量的影响差异有统计学意义(F+Gz(1,23)=22.37,P=0.0001);但暴露时间对兔hs-CRP含量不产生影响(F暴露时间(1,23)=0.82,P=0.4550);+Gz暴露与持续时间之间的交互效应对hs-CRP表达量的影响差异无统计学意义(F+Gz×暴露时间(1,1)=1.10,P=0.3540).主动脉电镜观察结果 也给予支持. 结论 重复、长期+Gz暴露可致兔血清hs-CRP长期处于高水平状态,提示应重视飞行员心血管损伤风险的预测.

Objective To investigate the repeated and long-term +Gz exposure caused highsensitivity C-reactive protein (hs-CRP) changes in rabbit's serum and find its indication for predicting cardiovascular risk. Methods Twenty-four male rabbits were randomly and averagely divided into 4-week-exposure group, 8-week-exposure group, 12-week-exposure group and 3 control groups that corresponding to 4, 8 and 12 weeks tests. Rabbits were exposed under +Gz 3 times a day with 5 min interval and exposures were repeated 3 times a week. The G load was started form +4 Gz for 20 s with 1 G/s onset rate at first week. Then the G level and duration increased by 0. 5 Gz and 5 s per week until +6 Gz and 40 s reached. When last exposure finished venous blood of rabbits was collected and hs-CRP was measured. Results Compared with control groups the effects of +Gz exposure on hs-CRP content were significant (F+Gz(1,23) = 22.37, P= 0. 0001) but on duration (Fduration(1,23) =0. 82,P=0.4550). The interact effects between +Gz exposure and duration on hs-CRP content were insignificant (F+G×duration(1,23) = 1.10, P= 0. 3540). What the microscope observed proved the statistic analysis. Conclusions Repeated and long-term +Gz exposure would lead to high serum hs-CRP content in rabbit. It implies that such change may be used for predicting cardiovascular risk and be hopefully applied for pilot.