褪黑素及iNOS在豚鼠形觉剥夺性近视眼的表达及作用

Expression and effect of melatonin and iNOS on FDM of guinea pig

目的 建立豚鼠形觉剥夺性近视眼(FDM)动物模型,观察视网膜中褪黑素和诱导型一氧化氮合酶(iNOS)在FDM中的变化及作用.方法 将出生7 d的30只豚鼠用半透明眼罩遮盖右眼,左眼作为对照,遮盖8周,然后检影验光检测双眼屈光度,A超测量双眼眼轴长度;免疫组化法分析褪黑素和iNOS的表达.结果 形觉剥夺使眼轴增长,近视屈光度增加,视网膜中褪黑素免疫活性降低而iNOS的活性增加(P＜0.05).结论 褪黑素和iNOS可能参与调控FDM的形成,褪黑素和iNOS之间呈负相关,也可能同时受其他因子的调控.

Objective Using a guinea pig model to explore the effects of retinal melatonin and iNOS on FDM.Methods Thirty guineas 7 day - old, their right eyes were worn with monocular translucent goggles for 8 weeks, the left eyes were contral eyes.The axial length was measured with A - mode ultrasound, and retino -scopic refraction was performed.Melatonin and iNOS was analysed with immunohistochemistry.Results The axial length of occluded eyes were significantly longer than control eyes (P ＜ 0.05).Form -deprivation eyes were significantly more myopia than control eyes(P ＜ 0.05).Form - deprivation reduced the immunostaining of melatonin, increased the immunostaining of iNOS.Conclusions Melatonin and iNOS may be involved in the positive regulation in FDM.The similar expression of melatonin and iNOS implied some interaction between the melatonin and iNOS, probably other factors also involve in the regulation procedure in FDM.