核转录因子-кB mRNA在乳腺癌中的表达

Expression of nuclear factor-кB in breast cancer

目的 探讨核转录因子(NF)-кB mRNA在乳腺癌组织中的表达及同临床病理指标之间的关系.方法 运用逆转录聚合酶链式反应技术测定70例乳腺癌及配对正常乳腺组织标本中转录因子-кB mRNA表达,分析其阳性表达率同临床病理指标的关系.结果 70例乳腺癌组织中44例呈NF-кB mRNA阳性表达,70例配对正常乳腺组织中29例阳性表达,乳腺癌组织中核转录因子-кB mRNA表达显著高于配对组织(P＜0.05);淋巴结转移组(73.9%)、临床晚期乳腺癌(84.6%)中NF-кB mRNA表达均显著高于无转移组(41.7%)及早期组(50.0%),P<0.05.结论 NF-кB mRNA在乳腺癌组织中高表达,同肿瘤转移及进展相关,NF-кB参与了乳腺癌转移,可预测肿瘤进展及预后.

Objective To explore the expression of nuclear factor-кB(NF-кB) mRNA and its relationship with clinical-pathological index in breast cancer. Methods Reverse transcription polymerase chain reaction was applied to detect NF-кB mRNA expression in 70 cases of breast cancer and normal paired tissues and the relationship between the positive rate of NF-кB and clinical-pathological index were analyzed. Results Forty-four cases of breast cancer were NF-кB mRNA positive in 70 cases of breast cancer which was significantly higher than 29 cases positive normal paired tissues(P＜0.05).Relatively the NF-кB mRNA positve rate in groups of lymph node metastasis(73.9%)and advanced stage(84.6%) were significantly higher than those in non- metastasis(41.7%)and early-stage(50.0%),P＜0.05.Conclusions NF-кB mRNA is highly expressed in breast cancer and correlate with tumor metastasis and progression. NF-кB plays a role in breast cancer progression and maybe predicate the advancement and prognosis of breast cancer.