环氧化酶-2与血管内皮生长因子D在乳腺癌中的表达及临床意义

Expression and clinical significance of COX-2 and VEGF-D in breast cancer

目的 探讨环氧化酶-2(COX-2) 和血管内皮生长因子D(VEGF-D)在乳腺癌中的表达及临床意义.方法 用免疫组化SP法检测59例乳腺癌及59例乳腺小叶增生病理组织中COX-2 和VEGF-D的表达,分析二者的表达情况与乳腺癌临床病理因素的关系.COX-2与VEGF-D在乳腺癌中的表达同年龄、肿块大小的关系用Wilcoxon秩和检验分析,COX-2与VEGF-D在乳腺癌中的表达同淋巴结转移及雌激素受体(ER)的关系用普通四格表卡方检验分析,COX-2与VEGF-D在乳腺癌中表达的相关性采用McNemar 配对χ2检验分析.结果 COX-2在乳腺癌和乳腺小叶增生组织中阳性表达率分别为61.0%(36/59)和11.9%(7/59),VEGF-D在乳腺癌和增生组织中的阳性表达率分别为74.6%(44/59)和8.5%(5/59),COX-2 和VEGF-D在乳腺癌及乳腺小叶增生组织中的表达比较差异有统计学意义(P<0.05).在乳腺癌组织中,COX-2 和VEGF-D的表达呈正相关(P<0.05);COX-2的表达同淋巴结转移呈正相关(P<0.05),同ER表达呈负相关(P<0.05);VEGF-D的表达同淋巴结转移呈正相关(P<0.05).结论 COX-2和VEGF-D高表达是乳腺癌侵袭转移的重要生物学标志.

Objective To investigate the expression of cyclooxygenase-2(COX-2)and vascular endothelial growth factor -D(VEGF-D) in human breast carcinoma and to investigate the relationships of COX-2 and VEGF-D with clinical and pathological characteristics.Methods The expression of COX-2 and VEGF-D were detected by immunohistochemistry SP technique in 59 cases of breast cancer and 59 cases of hyperplasia tissue.The relationship between the expression of COX-2,VEGF-D in breast cancer and the age, the volume was analyzed with Wilcoxon rank sum test.The relationship between the expression of COX-2, VEGF-D in breast cancer and the expression of estrogen receptor(ER) and lymph node metastasis was analyzed with Chi-square test. The relationship between the expression of COX-2 and VEGF-D in breast cancer was analyzed with Matching fourfold table.Results The positive expression rates of COX-2 were 61.0%(36/59) in breast cancer,and 11.9% (7/59) in hyperplasia.The positive expression rates of VEGF-D were 74.6%(44/59) in breast cancer,and 8.5%(15/59)in hyperplasia.The expression of COX-2 and VEGF-D in two groups had statistical differences(P<0.05). A significant positive correlation was found between the expression of VEGF-D and COX-2 in breast cancer(P<0.05). The expression of COX-2 in breast cancer was positively correlated with lymph node metastasis and negatively with the expression of ER (P<0.05). The expression of VEGF-D was positively correlated with lymph node metastasis(P<0.05). Conclusions COX-2 and VEGF-D are important biological markers for invasion and metastasis in breast cancer.