Hp感染与胃癌变过程中端粒酶逆转录酶和细胞增殖核抗原的关系

Relationship between Helicobacter pylori infection and expressions of TERT and PCNA during gastric carcinomatous change

目的 探讨胃癌及癌前病变组织中端粒酶逆转录酶(TERT)和细胞增殖核抗原(PCNA)的表达及其临床意义,并分析幽门螺杆菌(Hp)感染与端粒酶逆转录酶(TERT)和细胞增殖核抗原表达的关系.方法 采用Warthin-Starry(W-S)法、原位杂交法及免疫组化技术检测127例经病理证实的不同胃黏膜病变中Hp感染、TERT mRNA、PCNA的表达.结果 胃癌组PCNA和TERT mRNA阳性率均分别高于慢性萎缩性胃炎、肠上皮化生、异型增生(P＜0.05);Hp阳性组PCNA和TERT表达均高于Hp阴性组(P＜0.01).结论 Hp可通过刺激胃黏膜细胞过度增殖,同时诱导端粒酶活化和PCNA异常表达,从而启动和加速胃癌的发生发展过程.PCNA、TERT的过度表达与胃癌的发生有关,可作为筛选胃癌高危癌前病变的标志物.

Objective To investigate the expressions of cell proliferation of nuclear antigen (PCNA) and telomerase reverse transcriptase (TERT) mRNA and their clinical significance ingastri precance-rous lesion and carcinoma.To analyse the relationship between Helicobacter pylori infection and expressions of TERT and PCNA. Methods Expressions of Hp,TERT mRNA and proliferating cell nuclear antigen in 127 cases of different gastric pathological changes were respectively determined by in situ hybridization,Warthin-Starry and immunohistochemistry staining. Results The positive staining rates of TERT mRNA and PCNA in GC were respectively higher than those in gastric precancerous lesion group (P＜0.05). In Hp positive group, the expression of TERT mRNA and PCNA were higher than that in Hp negative group(P＜0.01). Conclusions Helicobacter pylori can stimulate gastric mucosa to excessive proliferate,at the same time,can induce the telomerase to activate and the disorder expression of PCNA, thereby, priming and accelerating the pathogenesis and development of gastric carcinoma.The excessive expression of PCNA and TERT are related to gastric carcinoma.Using them to screening high risk of precancerous lesion of gastric carcinoma.