辛伐他汀对人结肠癌裸鼠移植瘤血管生成的影响

Effects of simvastatin on the angiogenesis of colonic carcinoma xenograft in nude mice

目的 探讨辛伐他汀对结肠癌裸鼠移植瘤血管生成的影响.方法 以人结肠癌colo-320细胞注入12只雌性裸鼠皮下建立移植瘤模型,随机分为两组.治疗组每日口服辛伐他汀10 mg/kg,对照组给予同体积PBS,28 d后记录肿瘤体积,应用免疫组织化学法检测两组肿瘤组织C-fos、血管内皮生长因子(VEGF)表达及微血管密度(MVD).结果 治疗组肿瘤体积(1.29±0.14)cm3显著小于对照组的(2.07±0.18)cm3(P<0.05), C-fos、VEGF及MVD在治疗组分别为2.8±0.7,2.7±0.9,12.7±1.1,显著低于对照组的5.8±1.7,4.7±1.5,20.7±1.5 (P<0.05).结论 辛伐他汀可能通过抑制C-fos、减少VEGF 的表达抑制结肠癌裸鼠移植瘤血管生成.

Objective To investigate the effects of simvastatin on the angiogenesis of colonic carcinoma xenografts in nude mice. Methods Twelve female athymic mice inoculated with human colonic cancer cell line colo-320 subcutaneously were randomly divided into two groups. The treatment group were administrated with simvastatin orally at a dose of 10 mg/kg. The control group were given the same volume of PBS. After 28 days tumor volume were recorded. C-fos, VEGF expression and microvascular density (MVD) of the xenograft tissue were detected by immunohistochemical staining. Results The volume of grafting tumour in treatment group (1.29±0.14)cm3 were significantly lower than those in control group (2.07±0.18)cm3 (P<0.05). C-fos,VEGF and MVD were 2.8±0.7,2.7±0.9,12.7±1.1 in carcinoma tissues treated with simvastatin, which were lower than those(5.8±1.7,4.7±1.5,20.7±1.5,respectively) in control group (P<0.05). Conclusions Simvastatin may suppress angiogenesis of colonic carcinoma xenografts in nude mice via inhibiting C-fos and reducing the expression of VEGF.