摘要
目的应用纳米金磁微粒标记抗人前列腺干细胞抗原(PSCA)单抗7F5,构建PSCA特异性MR分子探针7F5@GoldMag,检测其理化性质和免疫活性。方法将金磁微粒作为MR对比剂标记7F5,应用非共价键偶联方法构建PSCA特异性MR分子探针7F5@GoldMag,计算偶联效率;采用激光共聚焦显微镜观察、流式细胞术、透射电镜观察等方法,检测探针与前列腺癌PC-3细胞结合的特异性。结果成功构建了PSCA特异性MR分子探针7F5@GoldMag。流式细胞术检测结果:7F5@GoldMag与PC-3细胞结合率为92.1%;激光共聚焦显微镜观察见PC-3细胞与7F5@GoldMag有特异性结合,红色荧光位于细胞膜;透射电镜观察PC-3近细胞膜处可见多个团块状致密电子密度颗粒聚集。结论 PSCA特异性MR分子探针7F5@GoldMag可与前列腺癌PC-3细胞特异性结合。
Objective To construct the PSCA-specific MR molecular probe 7F5@GoldMag,and to reveal its biochemical characteristics. Methods The PSCA-specific MR molecular probe 7F5@GoldMag was constructed by noncovalent bond conjugating anti-human PSCA monoclonal antibody (mAb) 7F5 with GoldMagTM-CS nanoparticles,and the coupling efficiency was calculated. The specific binding capability for 7F5@GoldMag to prostate cancer cells was defined by using laser confocal microscopy,transmission electron microscope,and flow cyto...
出处
《国际医学放射学杂志》
2011年第1期4-8,共5页
International Journal of Medical Radiology
基金
国家自然科学基金(项目批准号:30973408/H1608)
关键词
前列腺癌
前列腺干细胞抗原
单克隆抗体
纳米金磁微粒
磁共振成像
分子成像
Prostate carcinoma
Prostate stem cell antigen
Monoclonal antibody
GoldMag nanoparticles
Magnetic resonance imaging
Molecular imaging
